Effects of elevated CO2 and/or O3 on growth, development and physiology of wheat (Triticum aestivum L.)

Abstract

AbstractTwo cultivars of spring wheat (Triticum aestivum L. cvs. Alexandria and Hanno) and three cultivars of winter wheat (cvs. Riband, Mercia and Haven) were grown at two concentrations of CO2 [ambient (355 pmol mol−1) and elevated (708 μmol mol−1)] under two O3 regimes [clean air (< 5 nmol mol−1 O3) and polluted air (15 nmol mol−1 O3 at night rising to a midday maximum of 75 nmol mol−1)] in a phytotron at the University of Newcastle‐upon‐Tyne. Between the two‐leaf stage and anthesis, measurements of leaf gas‐exchange, non‐structural carbohydrate content, visible O3 damage, growth, dry matter partitioning, yield components and root development were made in order to examine responses to elevated CO2 and/or O3.Growth at elevated CO2 resulted in a sustained increase in the rate of CO2 assimilation, but after roughly 6 weeks' exposure there was evidence of a slight decline in the photosynthetic rate (c.‐15%) measured under growth conditions which was most pronounced in the winter cultivars. Enhanced rates of CO2 assimilation were accompanied by a decrease in stomatal conductance which improved the instantaneous water use efficiency of individual leaves. CO2 enrichment stimulated shoot and root growth to an equivalent extent, and increased tillering and yield components, however, non‐structural carbohydrates still accumulated in source leaves. In contrast, long‐term exposure to O3 resulted in a decreased CO2 assimilation rate (c. ‐13%), partial stomatal closure, and the accumulation of fructan and starch in leaves in the light. These effects were manifested in decreased rates of shoot and root growth, with root growth more severely affected than shoot growth.In the combined treatment growth of O3‐treated plants was enhanced by elevated CO2, but there was little evidence that CO2 enrichment afforded additional protection against O3 damage. The reduction in growth induced by O3 at elevated CO2 was similar to that induced by O3 at ambient CO2 despite additive effects of the individual gases on stomatal conductance that would be expected to reduce the O3 flux by 20%, and also CO2‐induced increases in the provision of substrates for detoxification and repair processes. These observations suggest that CO2 enrichment may render plants more susceptible to O3 damage at the cellular level. Possible mechanisms are discussed.