Effects of elevated CO2 and temperature on leaf characteristics, photosynthesis and carbon storage in aboveground biomass of a boreal bioenergy crop (Phalaris arundinacea L.) under varying water regimes

Abstract

This work examined the effects of elevated CO2 and temperature and water regimes, alone and in interaction, on the leaf characteristics [leaf area (LA), specific leaf weight (SLW), leaf nitrogen content (NL) based on LA], photosynthesis (light-saturated net carbon fixation rate, Psat) and carbon storage in aboveground biomass of leaves (Cl) and stem (Cs) for a perennial reed canary grass (Phalaris arundinacea L., Finnish local cultivar). For this purpose, plants were grown under different water regimes (ranging from high to low soil moisture) in climate-controlled growth chambers under the elevated CO2 and/or temperature (following a factorial design) over a whole growing season (May–September in 2009). The results showed that the elevated temperature increased the leaf growth, photosynthesis and carbon storage of aboveground biomass the most in the early growing periods, compared with ambient temperature. However, the plant growth declined rapidly thereafter with a lower carbon storage at the end of growing season. This was related to the accelerated phenology regulation and consequent earlier growth senescence. Consequently, the elevation of CO2 increased the Psat, LA and SLW during the growing season, with a significant concurrent increase in the carbon storage in aboveground biomass. Low soil moisture decreased the Psat, leaf stomatal conductance, LA and carbon storage in above ground biomass compared with high and normal soil moisture. This water stress effect was the largest under the elevated temperature. The elevated CO2 partially mitigated the adverse effects of high temperature and low soil moisture. However, the combination of elevated temperature and CO2 did not significantly increase the carbon storage in aboveground biomass of the plants.