Effects of elevated CO2 and N on tree–grass interactions: an experimental test using Fraxinus excelsior and Dactylis glomerata

Abstract

1 The invasion of grasslands by woody species is often associated with changes in environmental conditions, but few studies have addressed the impact of climate change on the competitive interactions between tree seedlings and herbaceous vegetation. We examined patterns of growth and morphology in Fraxinus excelsior seedlings germinating in the presence or absence of grass competition (Dactylis glomerata) at either low (380 p.p.m.) or high (645 p.p.m.) atmospheric carbon dioxide (CO2), and at two levels of nitrogen (N) nutrition. 2 Elevated CO2 had a positive effect on Fraxinus dry mass irrespective of N treatment, but the magnitude of growth response was small. In contrast, Dactylis dry mass showed a significant CO2 × N interaction: no apparent response to elevated CO2 in the low N treatment compared with a 25% dry mass increase in the high N, high CO2 treatment. 3 Dactylis and Fraxinus showed greater responses to N compared with CO2 in terms of dry mass, morphology and biomass allocation. The direction of Fraxinus responses to N varied depending on the trait examined and the grass competition treatment. 4 Both the competitive intensity (Cint) and the importance of the competition (Cimp) experienced by Fraxinus seedlings increased with an increase in N availability. Contrary to expectations, elevated CO2 had no significant effect on either Cint or Cimp. 5 Plant plasticity may have important implications for the long-term success of tree seedlings in grasslands. Our results suggest that the combination of both grass and tree seedling responses to CO2 may have indirect benefits for the persistence of woody invaders in high-nutrient grasslands under future atmospheric conditions.