Effects of electron acceptors, reducing agents, and toxic metabolites on anaerobic degradation of heterocyclic compounds

Abstract

Degradation of four heterocyclic compounds was examined under nitrate-reducing, sulphate-reducing and methanogenic conditions. Soil samples from a creosote-polluted site in Denmark were used as inoculum. Indole and quinoline were degraded under all redox conditions with the highest degradation rates obtained under sulphate-reducing conditions. Benzothiophene and benzofuran were not degraded during the observation period of 100 days under any of the redox conditions. Indole and quinoline degrading cultures could be repeatedly transferred under all redox conditions, except for degradation of quinoline under sulphate-reducing conditions which was inhibited by sulphide at concentrations above 0.8 mM. Degradation of quinoline under methanogenic conditions was also inhibited by 3.2 mM sulphide used as a reducing agent, but sulphide had no inhibitory effect on the degradation of indole in methanogenic and sulphate-reducing soil slurries.