Abstract

The proteolysis of Eight Holstein steers, were slaughtered. ES of low voltage (40V, was done one pairs of steers for 30, 60, and 90 sec, respectively within 5 min after slaughter. At 3 hr post-mortem, samples of Biceps femoris muscles were obtained. Each muscle was packed and stored at 1±1 o C within 3 or 4 hr after slaughter. Muscles were then analyzed at 0, 3, 5, 7, 14, and 21 days. After storage. SDS-PAGE of the myofibrillar proteins was performed Warner-Bratzler shear force values were measured

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