Effects of Eggshell Membrane on Keratinocyte Differentiation and Skin Aging In Vitro and In Vivo.

Kyohei Furukawa,Yukio Hasebe,Masaya Kono,Hisanori Kato,Tetsuro Kataoka,Huijuan Jia

doi:10.3390/nu13072144

Abstract

Skin aging is one of the hallmarks of the aging process that causes physiological and morphological changes. Recently, several nutritional studies were conducted to delay or suppress the aging process. This study investigated whether nutritional supplementation of the eggshell membrane (ESM) has a beneficial effect on maintaining skin health and improving the skin aging process in vitro using neonatal normal human epidermal keratinocytes (NHEK-Neo) and in vivo using interleukin-10 knockout (IL-10 KO) mice. In NHEK-Neo cells, 1 mg/mL of enzymatically hydrolyzed ESM (eESM) upregulated the expression of keratinocyte differentiation markers, including keratin 1, filaggrin and involucrin, and changed the keratinocyte morphology. In IL-10 KO mice, oral supplementation of 8% powdered-ESM (pESM) upregulated the expression of growth factors, including transforming growth factor β1, platelet-derived growth factor-β and connective tissue growth factor, and suppressed skin thinning. Furthermore, voltage-gated calcium channel, transient receptor potential cation channel subfamily V members were upregulated by eESM treatment in NHEK-Neo cells and pESM supplementation in IL-10 KO mice. Collectively, these data suggest that ESM has an important role in improving skin health and aging, possibly via upregulating calcium signaling.

Highlights

As pESM contains 91~94% indigestible protein and approximately 46% of them are digested and absorbed in the digestive tract [8], we tested the effects of three types of eggshell membrane (ESM), pESM, hydrolyzed ESM (hESM) and enzymatically hydrolyzed ESM (eESM), on cytotoxicity and cell morphology in cell culture experiments
The value was significantly decreased by 1 mg/mL of pESM, 2 and 3 mg/mL of hESM, and 1–3 mg/mL of eESM in NHEK-neo cells
The present study indicated that eESM induces keratinocyte differentiation in NHEKNeo cells and that pESM supplementation improves the decline in skin thickness in IL-10

Summary

Introduction

Physiology and function [2], increases disease morbidity and mortality [3] and decreases the health-related quality of life (HRQoL). One of the hallmarks of the aging process, leads to skin wrinkling, hair graying, scalp hair thinning/baldness, thin skin, etc. These physiological changes are caused by the accumulation of macromolecular damage, impairment of tissue renewal, loss of physiological function integrity [4]. Skin aging is mainly divided into two categories; chronological aging and photo-aging [5]. Chronological aging is occurred by internal factors (such as hormone levels, genotypes, etc.) and is hard to regulate, as these factors naturally change with age

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Results

Conclusion