Effects of edaravone on the expression of lung transforming growth factor-β1 in dogs with cardio-pulmonary bypass

Abstract

Objective To study the effects of edaravone (ED) on the expression of lung transforming growth factor-β1 (TGF-β1) in dogs with cardio-pulmonary bypass (CPB).Methods Thirty adult dogs were randomly divided into three groups (n =10):group C receiving normal saline during CPB,group E1 receiving ED before CPB,and group E2 receiving ED during CPB.Lung superoxide dismutase (SOD) activity,malondialdehyde (MDA) level,and TGF-β1 expression were determined before CPB (T0),30 min during CPB (T1),15 rmin after CPB (T2),30 min after CPB (T3),60 min after CPB (T4) in all dogs.The histological pathology of lung was evaluated with light microscope.Arterial blood samples were collected for the monitoring of respiratory index (RI) at T0,T3 and T4.Results Compared with T0,RI increased at T3 and T4 in all groups(P＜0.05).RI was higher in group C [(4.66±0.71),(5.19±0.43)] than group E1 [(1.21±0.39),(1.33±0.51)] or E2 [(1.87±0.53),(2.31±0.67)] and was higher in group E2 than group E1(P＜0.05).Compared with T0,SOD activity decreased gradually at T1-T4 in all groups (P＜0.05).SOD activity was higher in groups E1[(10.2±1.1),(9.9±1.5),(9.8±1.1),(10.2±1.1) U/mg] and E2 [(10.4±1.2),(10.0±1.1),(9.9±1.2),(10.2±1.1) U/mg] than group C[(8.4±1.2),(8.1±1.7),(8.0±1.2),(8.3±1.3) U/mg] at T1-T4 (P＜0.05).Compared with T0,MDA level increased gradually at T1-T4 (P＜0.05).MDA level was lower in groups E1 and E2 than group C at T1-T4(P＜0.05).Histopathological observation:group C showed severe inflammatory responses that were alleviated in groups E1 and E2.The expression of TGF-β1 was ocassionally detected at T0,and increased at T1-T4 in all groups.TGF-β1 expression was lower in group E1 [(169.6±1.1),(183.9±1.1),(173.8±0.9),(186.0±0.7)] than groups E2 [(150.4±1.1),(166.2±1.0),(151.7±0.8),(153.2±0.8)] and C [(143.2±1.2),(140.3±0.8),(133.3±0.7),(146.4±0.8)](P＜0.05).Conclusions CPB increased lung TGF-β1 expression and injury,which might be improved by ED.ED used before CPB provided better protection than used during CPB. Key words: Edaravone; Transforming growth factor-β1; Cardiopulmonary bypass; Lung injury; Dog