Effects of Early Transplantation of the Faecal Microbiota from Tibetan Pigs on the Gut Development of DSS-Challenged Piglets.

H Diao,H L Yan,J Yu,X B Mao,J He,P Zheng,D W Chen,Y Xiao,B Yu

doi:10.1155/2021/9823969

Abstract

The present study was conducted to investigate the effects of early transplantation of the faecal microbiota from Tibetan pigs on the gut development of dextran sulphate sodium- (DSS-) challenged piglets. In total, 24 3-day-old DLY piglets were divided into four groups (n = 6 per group); a 2 × 2 factorial arrangement was used, which included faecal microbiota transplantation (FMT) (from Tibetan pigs) and DSS challenge. The whole trial lasted for 55 days. DSS infusion increased the intestinal density, serum diamine oxidase (DAO) activity, and colonic Escherichia coli count (P < 0.05), and decreased the Lactobacillus spp. count and mRNA abundances of epidermal growth factor (EGF), glucagon-like peptide-2 (GLP-2), insulin-like growth factor 1 (IGF-1), occludin, mucin 2 (MUC2), regeneration protein IIIγ (RegIIIγ), and interleukin-10 (IL-10) in the colon (P < 0.05). FMT increased the Lactobacillus spp. count and mRNA abundances of GLP-2, RegIIIγ, and IL-10 in the colon (P < 0.05), and decreased the intestinal density, serum DAO activity, and colonic E. coli number (P < 0.05). In addition, in DSS-challenged piglets, FMT decreased the disease activity index (P < 0.05) and attenuated the effect of DSS challenge on the intestinal density, serum DAO activity, and colonic E. coli number (P < 0.05). These data indicated that the faecal microbiota from Tibetan pigs could attenuate the negative effect of DSS challenge on the gut development of piglets.

Highlights

Over thousands of years of evolution, hosts and bacteria have developed beneficial relationships, creating a mutually beneficial symbiotic environment [1]
Colitis is a common gastrointestinal dysfunction disease, and it is clinically manifested as diarrhoea and bloody stools, which are associated with an abnormal immune response induced by intestinal microbiome disorders [31]
We found that faecal microbiota transplantation (FMT) from Tibetan pigs significantly reduced the Disease Activity Index (DAI) in the dextran sulphate sodium- (DSS-)challenged piglets, indicating that early FMT from Tibetan pigs can alleviate the clinical symptoms induced by DSS infusion

Summary

Introduction

Over thousands of years of evolution, hosts and bacteria have developed beneficial relationships, creating a mutually beneficial symbiotic environment [1]. Hosts provide a basic developmental environment for microorganisms [2, 3]. Many studies in the biomedical field have shown that the intestinal microbiota are closely related to host health; these microorganisms can affect the digestion, absorption, and metabolism of nutrients, and regulate the physiological functions and the occurrence and development of diseases in hosts [4, 5]. The gut microbiota can be used as targets for regulating metabolism and treating intestinal diseases in hosts [12]. Transplanting faecal materials from healthy individuals into patients with different diseases, such as inflammatory bowel disease, Clostridium difficile infection, metabolic diseases, and immune disorders, can be beneficial for the treatment of these diseases [13,14,15,16]

Methods

Results

Conclusion