Effects of Dual Microalgal Species Biofilms on New Zealand Black-Footed Abalone (Hailotis iris) Larval/Post-Larval Processes

Abstract

Dual microalgal species biofilms with distinct amino acid characteristics were tested for their effects on abalone (Haliotis iris) initial attachment, metamorphosis, settlement, and survival. Nine microalgal species, isolated from around Auckland, New Zealand, were grown in the laboratory, and used to produce nine dual microalgal species biofilms. Abalone larvae were exposed to the different biofilm treatments and controls (no biofilms) for seven (attachment and metamorphosis) and 14 (settlement and survival) days. The larvae performed significantly better in the dual microalgal biofilms compared to controls, and some biofilms resulted in better attachment, metamorphosis, settlement, and survival than others. For example, the best-performing biofilms almost always contained cyanobacteria (Oscillatoria cf. erythrata, Anacystis cf. aeruginosa, and Schizothrix cf. calcicola), which are attributed with better nutritional values. However, the amino acid profiles did not produce a clear pattern with regard to their effects on the four larval processes. Instead, total amino acid (TAA) content was positively correlated with percentage of attachment and metamorphosis. Greater TAA contents are likely to reflect greater amounts of extra-cellular polymers within the biofilms, which are suggested to improve larval performance.