Effects of DPSS laser on Human Dermal Fibroblasts and Human Breast Cancer cells lines

Abstract

Different types of lasers are used in various research and medical applications such as therapies, surgical procedures and medical diagnostic. Low level laser therapy (LLT) utilizes low intensity power with various wavelength that are proposed for a wide variety of medical applications for promoting tissue regeneration, reduce inflammation and pain, and wound healing process. This study aimed to investigate the effect of laser irradiation diode-pumped solid state laser (DPSS) violet 405nm on cell viability of human normal and cancer cell lines. Human dermal fibroblasts (HDF) and human breast cancer (MCF7) cells lines were exposed to laser light for 5 minutes of exposure time. The cells viability were analyzed by Trypan Blue Exclusion Assay and morphological changes were evaluated using inverted microscope. Findings revealed that the viability of HDF was not affected by laser irradiation. However, this laser treatment exhibited cytotoxic effect on MCF7. Reduction of cells number and changes in cell morphology were observed in MCF7 after being treated with laser in short duration of time. Thus, the results may suggest that DPSS violet laser at 405nm had inhibition effect on cancer cell lines and did not cause loss of cell viability in normal cell lines.