Abstract
BackgroundIn order to understand and regulate complex genetic networks in living cells, it is important to build simple and well-defined genetic circuits. We designed such circuits using a synthetic biology approach that included mathematical modeling and simulation, with a focus on the effects by which downstream reporter genes are involved in the regulation of synthetic genetic circuits.ResultsOur results indicated that downstream genes exert two main effects on genes involved in the regulation of synthetic genetic circuits: (1) competition for regulatory proteins and (2) protein degradation in the cell.ConclusionsOur findings regarding the effects of downstream genes on regulatory genes and the role of impedance in driving large-scale and complex genetic circuits may facilitate the design of more accurate genetic circuits. This design will have wide applications in future studies of systems and synthetic biology.
Highlights
In order to understand and regulate complex genetic networks in living cells, it is important to build simple and well-defined genetic circuits
Our findings regarding the effects of downstream genes on regulatory genes and the role of impedance in driving large-scale and complex genetic circuits may facilitate the design of more accurate genetic circuits
Our results showed the effects of downstream reporter genes on regulatory genes of synthetic genetic circuits
Summary
In order to understand and regulate complex genetic networks in living cells, it is important to build simple and well-defined genetic circuits. We designed such circuits using a synthetic biology approach that included mathematical modeling and simulation, with a focus on the effects by which downstream reporter genes are involved in the regulation of synthetic genetic circuits. Synthetic genetic circuits are often designed such that they only comprise regulatory genes in synthetic genetic circuits, and do not include downstream genes, such as reporter genes or genes present in natural genetic circuits (Figure 1a) [1,2,3,4,5]. The first involves increased competition for regulatory proteins among the existing regulatory gene
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