Effects of down-regulating ornithine decarboxylase upon putrescine-associated metabolism and growth in Nicotiana tabacum L.

Heidi L Dalton,Alan D Neale,Cecilia K Blomstedt,John D Hamill,Kathleen D Deboer,Ros Gleadow

doi:10.1093/jxb/erw166

Abstract

Transgenic plants of Nicotiana tabacum L. homozygous for an RNAi construct designed to silence ornithine decarboxylase (ODC) had significantly lower concentrations of nicotine and nornicotine, but significantly higher concentrations of anatabine, compared with vector-only controls. Silencing of ODC also led to significantly reduced concentrations of polyamines (putrescine, spermidine and spermine), tyramine and phenolamides (caffeoylputrescine and dicaffeoylspermidine) with concomitant increases in concentrations of amino acids ornithine, arginine, aspartate, glutamate and glutamine. Root transcript levels of S-adenosyl methionine decarboxylase, S-adenosyl methionine synthase and spermidine synthase (polyamine synthesis enzymes) were reduced compared with vector controls, whilst transcript levels of arginine decarboxylase (putrescine synthesis), putrescine methyltransferase (nicotine production) and multi-drug and toxic compound extrusion (alkaloid transport) proteins were elevated. In contrast, expression of two other key proteins required for alkaloid synthesis, quinolinic acid phosphoribosyltransferase (nicotinic acid production) and a PIP-family oxidoreductase (nicotinic acid condensation reactions), were diminished in roots of odc-RNAi plants relative to vector-only controls. Transcriptional and biochemical differences associated with polyamine and alkaloid metabolism were exacerbated in odc-RNAi plants in response to different forms of shoot damage. In general, apex removal had a greater effect than leaf wounding alone, with a combination of these injury treatments producing synergistic responses in some cases. Reduced expression of ODC appeared to have negative effects upon plant growth and vigour with some leaves of odc-RNAi lines being brittle and bleached compared with vector-only controls. Together, results of this study demonstrate that ornithine decarboxylase has important roles in facilitating both primary and secondary metabolism in Nicotiana.

Highlights

Terrestrial plants have been subjected to herbivory since their emergence onto land ca. 450 million years ago and a wide array of physical and chemical defence systems have evolved to provide protection and facilitate their reproduction in native environments (Labandeira, 1998; Wellman and Gray, 2000)
Transcriptional and biochemical differences associated with polyamine and alkaloid metabolism were exacerbated in odc-RNA interference (RNAi) plants in response to different forms of shoot damage
Concentrations of polyamines in both leaves and roots, and tyramine in roots, were significantly lower in non-wounded odc-RNAi transgenic plants compared with corresponding tissues of non-wounded VC plants (Fig. 2A–D)

Summary

Introduction

Terrestrial plants have been subjected to herbivory since their emergence onto land ca. 450 million years ago and a wide array of physical and chemical defence systems have evolved to provide protection and facilitate their reproduction in native environments (Labandeira, 1998; Wellman and Gray, 2000). 450 million years ago and a wide array of physical and chemical defence systems have evolved to provide protection and facilitate their reproduction in native environments (Labandeira, 1998; Wellman and Gray, 2000). Alkaloids represent a diverse grouping of such chemical defences, with many thousands of chemical structures distributed widely across the plant kingdom (Aniszewshi, 2015). Biosynthesis of alkaloids generally involves the diversion of amino acid precursors from primary into secondary metabolism via the action of decarboxylases and is often enhanced by exposure of plants to biotic and/or abiotic stress conditions (Shoji and Hashimoto, 2013a). The genus Nicotiana (family Solanaceae) contains more than 75 species, native mainly to the Americas and mainland Australia, with representatives on South Pacific islands and in southern Africa (Knapp et al, 2004).

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Conclusion