Abstract
Protocorm-like bodies (PLB) obtained through in vitro culture of seedling leaf segment of Phalaenopsis cultivar ‘Surfrider’ × ( Phalaenopsis cultivar ‘Joseph Hampton’ × Doritaenopsis cultivar ‘Kaala Gleam’) were cultured on media containing peptone or tryptone at various concentrations. The addition of peptone and tryptone to the culture medium caused promoted growth of PLB. Maximum stimulation was obtained when 2 g l −1 of tryptone was added. The effects of dividing and size of PLB on the growth and organogenesis of PLB were examined. Segments from dorsal parts of PLB had a tendency to form plantlets, while segments from basal parts produced no plantlets, but a larger number of PLB than dosal segments. From the results of the growth and the PLB production rate, 1 4 B segments are recommended for use in proliferation of PLB.
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