Effects of distilled fluid of olfactory ensheathing cells by intracerebreventricular injection on treatment of Aizheimer's disease rat

Abstract

Objective To probe the effects of distilled fluid of olfactory ensheathing cells(DFOECs)by intracerebreventricular injection on treatment of Alzheimer's disease(AD)rat.Methods The model of AD rats were builded by intracerebreventficular injection of Aβ1-40.The rats were divided into the normal control groups,the model of AD ones,the manpower cerebral spinal fluid(MCSF)injected ones and the DFOECs injected ones at random,and there were eight rats every group.Four weeks alter being injected,the change of memory recovery was observed by Y maze test,the total number of nitric oxide synthase positive neurons and senile plaques of hippocampus were observed by NADPH-d and Congo red histochemical staining.Results ①Y msze test：TN.EN，D，TRT of DFOECs injected group obviously decreased compared to the model of AD group(P=0.000).The former was separately(18.82±2.64)times,(4.81±1.85)times,(5.18±4.86)s,(1236.78±97.26)s,the latter was (48.33±5.10)times,(10.83±2.98)times，(11.32±12.23)s，(2264.32±244.60)s.② NADPH-d histochemical staining:the total number of NOS positive neurons of DFOECs injected group were higher than that of the model of AD group(P=0.000),they were separately(16.08±2.51)entries/eye shot,(5.08±0.89)entries/eye shot.③Congo red histochemical staining：compared to the model of AD group and the DFOECs injected ones were significantly changed in SP-IA(P=0.000)，they were separately(481.15±144.18)，(74.39±9.14).④ There was not significant differences between the model of AD group and MCSF injected group.Conclusion The distilled fluid of olfactory ensheathing cells can greatly improve the effects on treatment of Alzheimer's disease rat by intracerebreventricular injection. Key words: Alzheimer's disease; Olfactory ensheathing cells; Distilled fluid