Abstract
Neuronal sensitivity to light stimulation can be a significant confounding factor for assays that use light to study neuronal processes, such as optogenetics and fluorescent imaging. While continuous one-photon (1P) blue light stimulation has been shown to be responsible for a decrease in firing activity in several neuronal subtypes, discontinuous 1P blue light stimulation commonly used in optogenetic experiments is supposed to have a negligible action. In the present report, we tested experimentally this theoretical prediction by assessing the effects produced by the most commonly used patterns of discontinuous 1P light stimulation on several electrophysiological parameters in brain slices. We found that, compared with continuous stimulation, the artefactual effect of light is reduced when discontinuous stimulation is used, especially when the duty cycle and light power are low.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
