Abstract
The (-)-anti-diol epoxide of benzo[c]phenanthrene (BPhDE) and (+)-anti-diol epoxide of benzo[a]pyrene (BPDE) were used to site-specifically modify oligonucleotides containing the binding site for the transcription factors AP-1 and TFIID, respectively. The modified oligonucleotides were incubated with the appropriate transcription factor and protein-DNA interaction analysed by the electrophoretic mobility shift assay. The available results show that a trans-BPhDE-N6-dA adduct located in the AP-1 binding sequence totally inhibits protein-DNA interaction. The presence of a trans-BPDE-N2-dG adduct near the TFIID binding site and thus, an expected bending of the helix, has no obvious effect on protein binding or the further assemblance of the transcription initiation complex.
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