Effects of differential protein transgelin-2 overexpression on biological characteristics of colon cancer cell lines

Abstract

Objective To study the effect of transgelin-2 expression on biological characteristics of colon cancer cells. Methods RT-PCR and Western blot were used to observe mRNA and protein expression of transgelin-2 in five colon cancer cell lines, screen for cell line with lower transgelin-2 expression; Transient transfection was performed to establish over-expression of transgelin-2 colon cancer cell line; The effect of transgelin-2 over-expression on the proliferation, apoptosis and the ability of migration and invasion of colon cancer cell were detected by CCK-8, low cytometric analysis and transwell method respectively. Results There was no statistical difference in proliferation and apoptosis in colon cancer cells with transgelin-2 over-expression compared with the controls; Enhanced ability of migration and invasion was found in colon cancer cells with transgelin-2 over-expression.After 15 hours culture in serum free medium, more transgelin-2 over-expressed colon cancer cells went through the transwell chamber bottom membrane than that in control group and empty vector transfected group 207±62 vs.114±29 vs. 120±26, F=7.302, P<0.05). After 24 hours, the differences remained statistically significant (179±32 vs. 95±33 vs. 95±28, F=10.960, P<0.05). Conclusions Transgelin-2 enhances migration and invasion ability of colon cancer cells. Key words: Colonic neoplasms; Neoplasm invasiveness; Transgelin-2