Effects of different Zinc levels in the sperm culture medium on sperm recovery and quality of sperms in the swim up procedure for sperm processing

Abstract

A controlled ill vitro study was carried out to observe the effect of different Zinc (Zn) levels on sperm recovery rate, chromosome integrity, cell membrane integrity and motility in the swim up procedure. Semen samples were obtained from males who underwent seminal fluid analysis at the Infertility Laboratory, Department of Obstetrics and Gynaecology, Faculty of Medicine, Ragama. Twenty normozoo­ spermic samples were randomly selected for the study and each sample was processed with supplemented Earl's Balanced Salt Solution (EBSS) containing different concentrations of Zn [0.5ml of supplemented EBSS with 251'1 of solution containing 0.61'mol (group 1) and 1.2 I'mol (group 2) of Zn respectively]. One aliquot processed with 25 1'1 of physiological saline with added EBSS served as the control. Pre and post wash sperm counts and motility were recorded immediately after processing. Post wash sperms from the three groups were observed for chromosome integrity, cell membrane integrity, and motility. Motility changes after four hours of incubation were also observed. The mean sperm concentration showed an increase in group 1 compared to the control sample [21.87 ± 21.61 (SO) millions / ml compared to 18.34 ± 19.73 millions / ml, P 0.05]. The mean differences in sperm concentration compared to the control showed statistically Significant differences in both groups where an increase was observed in group 1 [3.52 ± 4.96 (SO) millions / ml] and a reduction in group 2 (- 2.08 ± 6.59 millions/ ml).