Effects of different types of air supplementation on rainbow trout confined underwater

Abstract

Salmonid are farmed far offshore in a submersible cage in China, and air supplementation in the swim bladder is an important issue while the fish are confined in the submersed cage. The objective of this study is to test the effects of different types of air supplementation on the growth, energy budget, and fatty acid composition of the rainbow trout, Oncorhynchus mykiss, confined underwater. The fish were divided into four groups with four replicates (63.84 ± 2.03 g, 13 fish per tank): The control group fish (Ctrl) were cultured in a glass fiber tank with airstone; The mesh group (ME) used a net in a tank to keep the experimental fish from the water surface, and an airstone above the net; The airstone group (AM) added an airstone under the net; And the airdome group (DM) added an airdome under the net. At the end of a 35-day trial, the final mean weight (70.00 ± 1.63 g), percent weight gain (7.09 ± 0.96%) and survival rate (76.92 ± 0.00%) of the fish in ME group were significantly lower than those in Ctrl group (91.54 ± 5.19 g, 43.49 ± 10.35% and 100.00 ± 0.00%, respectively). The final mean weight of AM group was significantly lower than that of Ctrl group, whereas there were no significant differences in other growth indexes between control group, DM and AM group. The proportion of feeding energy allocated to growth in ME group was the lowest, the proportion in AM was significantly higher than ME group and lower than Ctrl group, and there was no significant difference between those in DM and Ctrl groups. The muscle saturated fatty acid level in Ctrl group was significantly lower than those in other groups. In conclusion, the growth of rainbow trout confined underwater for 35 days will slow down due to lower proportion of feeding energy allocated to growth and higher proportion to respiration. The addition of an airdome or airstone can partially compensate for the growth of the fish confined underwater long time. Rainbow trout confined underwater can partially compensate for the loss of buoyancy through increasing the muscle saturated fatty acid content.