Abstract
The heat tolerance of biological ice nucleation activity (INA) depends on their types. Different temperature treatments may cause varying degrees of inactivation on biological ice nuclei (IN) in precipitation samples. In this study, we measured IN concentration and bacterial INA in snow samples using a drop freezing assay, and compared the results for unheated snow and snow treated at 40 °C and 90 °C. At a measured temperature of −7 °C, the concentration of IN in untreated snow was 100–570 L−1, whereas the concentration in snow treated at 40 °C and 90 °C was 31–270 L−1 and 2.5–14 L−1, respectively. In the present study, heat sensitive IN inactivated by heating at 40 °C were predominant, and ranged 23–78% of IN at −7 °C compared with untreated samples. Ice nucleation active Pseudomonas strains were also isolated from the snow samples, and heating at 40 °C and 90 °C inactivated these microorganisms. Consequently, different temperature treatments induced varying degrees of inactivation on IN in snow samples. Differences in the concentration of IN across a range of treatment temperatures might reflect the abundance of different heat sensitive biological IN components.
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