Effects of different storage media on the activity of human adipose-derived stem cell

Abstract

Objective To determine the proper storage environment by comparing the activity of adipose-derived stem cell (ADSC) at 10% PRP, 10% HS and 0.9% NaCl. Methods ADSC was isolated and cultured. Then the ADSC was stored in 10% PRP, 10% HS, and 0.9% Nacl at room temperature (RT, about 26 ℃) for 2 hours. After that the proliferation ability, survival rate and differentiation ability of the cells were detected. Finally storage media of ADSC was determined. Results Compared with 0.9% NaCl, ADSC showed a better proliferation and higher differentiation ability at 10% PRP and 10% HS; besides, the survival rates of two groups were higher than the group of 0.9% Nacl. Among these groups, the survive rate of 10% HS and 10% PRP were 78.08% and 75.68% respectively, while it was 62.34% in 0.9% NaCl group. Conclusions 10% HS is more suitable for short-term preservation of ADSC. Key words: Breast Beauty; Stem cells; Preservation, biological; Serum; Platelet-rich plasma; Adipose-derived mesenchymal stem cell