Abstract
The effects of NaCl, Na2SO4, Na2HPO4, and Na3C6H5O7 on the production of 3-hydroxybutyrate, polyhydroxybutyrate, and by-products by Burkholderia cepacia. Proper addition of Na3C6H5O7 can significantly promote the production of 3-hydroxybutyric acid and polyhydroxybutyrate. The concentration, productivity, and yield of 3-hydroxybutyrate were increased by 48.2%, 55.6%, and 48.3% at 16 mM Na3C6H5O7. The increases of 80.1%, 47.1%, and 80.0% in the concentration, productivity, and yield of polyhydroxybutyrate were observed at 12 mM Na3C6H5O7. Na2SO4 and Na2HPO4 also have positive effects on the production capacity of 3-hydroxybutyrate and polyhydroxybutyrate within a certain range of concentration. NaCl is not conducive to the improvement of fermentation efficiency. Compared with a single nitrogen source, a mixed nitrogen source is more conducive to enhancing the production of 3-hydroxybutyrate and polyhydroxybutyrate.
Highlights
The effects of NaCl, Na2SO4, Na2HPO4, and Na3C6H5O7 on the production of 3-hydroxybutyrate, polyhydroxybutyrate, and by-products by Burkholderia cepacia
Bioprocess. (2021) 8:64 synthesis requires a higher level of substrate purity and catalyst performance, which results in increased production costs
The sodium salts with different anions at a specific concentration were added to the fermentation medium before fermentation to study the effects of the type and initial concentration of sodium salts on PHB and 3-HB production by B. cepacia cells (Table 1)
Summary
The effects of NaCl, Na2SO4, Na2HPO4, and Na3C6H5O7 on the production of 3-hydroxybutyrate, polyhydroxybutyrate, and by-products by Burkholderia cepacia. Chemical or enzymatic depolymerization of PHB usually requires strict control of process conditions and has a relatively low yield (Wang and Liu 2014). The in vivo depolymerization of PHB can be carried out under mild conditions with a higher yield, and its production efficiency can be improved more by optimizing fermentation conditions and medium components. This method has a great development potential, which is of great significance for reducing production costs and technical difficulties. The theoretical total yield of 3-HB is 0.770 g/g glucose based on Eq (1): 3C6H6O6 → 4CH3CH(OH)CH2COOH + 2CO2 + 2H2O (1)
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