Effects of different proteases enzymatic extraction on the lipid yield and quality of Antarctic krill oil.

Linlin Wang,Yatao Ding,Zhihe Wang,Yali Rong,Feng Yang,Yuan Yuan,Wenzheng Shi

doi:10.1002/fsn3.1017

Abstract

This study was investigated the effects of six proteases (papain, compound proteinase, acidic protease, neutrase, pancreatin, and alcalase) on the lipid yield and quality of krill oil. The result shown that the krill oil extracted by alcalase and compound proteinase led to comparatively higher lipid yields (5.29% and 4.90%, respectively), Content of tocopherols and vitamin A, the content of omega‐3 polyunsaturated fatty acids (PUFAs) and phospholipids extracted by alcalase was relatively higher. Control and alcalase had comparatively higher concentration of astaxanthin. On the whole, compared with the extraction of solvent, enzymatic hydrolysis could improve the quality and the lipid yield of krill oil. Therefore, enzymatic hydrolysis could be used as a better method to extract krill oil.

Highlights

Antarctic krill (Euphausia superba) is a keystone species of Antarctic ecosystems, acting as major prey item for most of the megafauna such as baleen whales, squid, crabeater seals and brush‐tailed penguins, and other vertebrates (Cleary, Durbin, & Casas, 2018)
Observation can be drawn that the lipid yield of neutrase and compound protease was 4.73% and 4.90%, respectively, and there was no significant difference (p < 0.05). This indicates that neutrase, compound proteinase, and alcalase can be used as a good protease for extracting Antarctic krill oil
The contents of vitamin A and tocopherol obtained by enzymatic hydrolysis of alcalase were higher, which was 95.32 μg/100g and 39.28 mg/100g, respectively, lower than 29.39 mg/100 g of tocopherols and higher than 34.32 mg/100 g of vitamin A of the research of Xie et al (2018), and the contents of vitamin A and tocopherols were positively associated with lipid yield; these results provide date support for the selection of protease to hydrolyze

Summary

Introduction

Antarctic krill (Euphausia superba) is a keystone species of Antarctic ecosystems, acting as major prey item for most of the megafauna such as baleen whales, squid, crabeater seals and brush‐tailed penguins, and other vertebrates (Cleary, Durbin, & Casas, 2018). Antarctic krill has attracted increasing attention due to its huge biomass and potential fishery resources and its special status in the Antarctic marine ecosystem (Brierley, Fernandes, & Brandon, 2002; Nicol, 2000; Pakhomov, Atkinson, & Meyer, 2004). The total number of krill was estimated to be 379 million tons (Atkinson, Siegel, Pakhomov, Jessopp, & Loeb, 2009). Krill quickly degraded, resulting in unpleasant taste and color changes due to high enzyme activity. Krill contains high fluoride content in its shell. Human consumption of whole body krill is considered to be present safety issues. Krill is mostly used for feed and bait, only a small proportion used for human consumption. The use of krill as human food has so far been limited. The use of krill as human food has so far been limited. (Kim, Jung, Lee, Chun, & Kim, 2014)

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Conclusion