Abstract

Sugarcane (Saccharum officinarum), being an important cash crop in Belize, accounts for 60% of agricultural exports providing employment for thousands of Belizeans. This research was carried out to determine the effects of different Paclobutrazol (PBZ-Cultar) concentrations used in the culture media on in vitro multiplication of sugarcane variety CPCL99-4455. Three PBZ-Cultar concentrations were tested and compared with the control. The parameters evaluated to determine the effects of PBZ-Cultar were average height, number of dead leaves and multiplication coefficient. The plants from this experiment were planted in the acclimatization phase to determine if the use of PBZ-Cultar had any effect of the growth and development of the plants ex vitro. The results revealed that the culture medium supplemented with 0.08% PBZ-Cultar concentration had the best results both in the multiplication phase as well as the acclimatization phase.

Highlights

  • Raw sugar is derived from both sugarcane and sugar beet

  • The primary objective of this research is to determine the effects of Paclobutrazol (PBZ) – Cultar concentration on in vitro multiplication of sugarcane variety CPCL99-4455 in terms of height, number of dead leaves, as well as the multiplication coefficient and determine its effect if any, in the acclimatization phase

  • These analyses allowed for inferences to be made about the variation in the mean heights, the number of dead leaves and multiplication coefficient of sugarcane (Saccharum officinarum) variety CPCL99-4455

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Summary

Introduction

Brazil and India are the world’s two largest sugar producers. Together, they have accounted for over half the world’s sugarcane (Saccharum officinarum) production for the past 40 years (Taylor and Koo, 2012). Sugarcane is an important cash crop for Belize’s economy. This crop is a highly placed commodity in consumer products as it serves as a major source of edible processed sugars (Ali et al, 2008). The sugar industry of Belize is of fundamental social and economic importance to the country. Day by day increasing use of sugar and its relevant products have created a challenging situation for sugarcane researchers and growers (SIRDI, 2015)

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