Effects of different methods of acupuncture and moxibustion on functional constipation in rats:a comparative study

Abstract

To compare the effects of acupuncture, electroacupuncture (EA) and moxibustion on functional constipation in rats. Sixty male Sprague-Dawley rats were divided into a control group (n=8), a model group (n=11), a medication group (n=8), an acupuncture group (n=11), an EA group (n=11) and a moxibustion group (n=11) by random number table. The rats in the model group, medication group, acupuncture group, EA group and moxibustion group were treated with intragastric administration of loperamide hydrochloride for 6 days continuously to establish the functional constipation models, while equal volume of drinking water was administrated to rats in the control group at the same time. The rats in the acupuncture group, EA group and moxibustion group were respectively treated with acupuncture, EA and moxibustion at "Tianshu" (ST 25) and "Shangjuxu" (ST 37) one hour after intragastric administration; rats in the medication group were treated with intragastric administration of cisapride suspension. All the treatment was given once a day for 6 days. At the last day of intervention, the 24-hour food intake, stool quantity and its water content were measured in each group; the pushing rate of intestine was measured; the structure of colon tissue and acidic mucus in its mucous layer were observed by hematoxylin-eosin dyeing and alcian blue dyeing; the expression of stem cell factor (SCF) and c-kit mRNA was detected by real-time PCR. Compared with the control group, the 24-hour food intake and stool quantity were reduced in the model group (both P<0.01), and the water content of stool and pushing rate of intestine were reduced (both P<0.01); compared with the model group, the stool quantity and its water content were increased in the medication group, acupuncture group, EA group (P<0.05, P<0.01), which were not significantly different from those in the moxibustion group (both P>0.05). The pushing rate of intestine in each intervention group was increased (all P<0.01). The 24-hour food intake and stool quantity in the medication group were not significantly different from those in the acupuncture group, EA group and moxibustion group (all P>0.05), and the water content of stool was only reduced in the moxibustion group (P<0.01). The pushing rate of intestine in the acupuncture group and moxibustion group was lower than that in the medication group (both P<0.01), while that in the EA group was not significantly different from that in the medication group (P>0.05). The water content of stool in the moxibustion group was lower than that in the acupuncture group and EA group (both P<0.01). The pushing rate of intestine in the acupuncture group and moxibustion group was lower than that in the EA group (both P<0.01). The HE staining result indicated the structure of colon tissue was normal, complete and similar in each group; the alcian blue staining indicated the acidic mucosubstance in the model group was lower than that in the control group; compared with the model group, the acidic mucosubstance in the medication group, acupuncture group, EA group and moxibustion group was all increased. Compared with the control group, the expression of SCF and c-kit mRNA was reduced in the model group (both P<0.05); compared with the model group, the expression of SCF and c-kit mRNA was increased in the medication group, acupuncture group, EA group and moxibustion group (all P<0.05); compared with the moxibustion group, the expression of c-kit mRNA was reduced in the acupuncture group and EA group (both P<0.05). Acupuncture, EA and moxibustion all can play a positive regulative role on functional constipation in rats, in which EA has the best efficacy, followed by acupuncture.