Abstract
Used apple root-stock JM7 stem-tips as material to study different LED influence of different stages of tissue culture. The results showed that in the culture medium (bud induction medium LS + 4.0mg/L 6-BA + 0.2 mg/L NAA, proliferation medium LS + 2.5mg/L 6-BA + 0.1mg/L NAA , rooting medium 1/2 LS + 0.5mg/L NAA + 1.0mg/L IAA), with 30g/L sugar and 9g/L agar, pH 5.8, autoclave 121°C, 20 minuets. At the same culture conditions, 21±1°C temperature, 12 hours of light, 12 hours of dark and same illumination time. The LED treatments were better than the fluorescent light of the germination; LED r/b ratio 5:5, was the best on germination effect, the height of seedling was 1.84cm and 10 leaves on 50 days of cultivation; LED r/b ratio 6:4, was the best on the proliferation stage and rooting effect stage, the height of seedling was 4.15cm on 45 days of proliferation and the main-root length was 5.97cm, and the height of seedling was 6.38cm on 45 days of rooting stage; followed by LED r/b 7:3 and 5:5. In conclusion LED treatments were advantageous to the germination, seedling differentiation and rooting of apple root-stock JM7 tissue culture, better than fluorescent lamp group. It could get more quantities and stronger plants in the same culture conditions, also saved the culture time. LED was cold light source, it could produce less excess heat and truly reflects the feature of power-saving effectively. So it was reduced of the tissue culture production in a certain extent.
Highlights
Used apple root-stock JM7 stem-tips as material to study different LED influence of different stages of tissue culture
On the 30 days and 50 days, the diameter of seedling height and true leaf diameter of LED treatments were significantly larger than the fluorescent lamp treatment
The results showed that, in the same conditions of apple root-stock“JM7”tissue culture, the LED lights were better than the fluorescent light source for whole culture process, the germination, proliferation, differentiation, and rooting were all better
Summary
Used apple root-stock JM7 stem-tips as material to study different LED influence of different stages of tissue culture. In conclusion LED treatments were advantageous to the germination, seedling differentiation and rooting of apple root-stock JM7 tissue culture, better than fluorescent lamp group. It could get more quantities and stronger plants in the same culture conditions, saved the culture time. LED was cold light source, it could produce less excess heat and truly reflects the feature of power-saving effectively It was reduced of the tissue culture production in a certain extent. The rapid propagation of apple root-stock by tissue culture method had achieved successful in many varieties [1,2,3,4], for example, G256, M9, Malus rockii Rehder, Zhongzhen, Qinzhen, Malus Teli and so on. It will use in the airborne dimming technology, food analysis and in the arctic circle vegetable cultivation use the LED lights with hydroponics technology
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