Effects of Different Inducer on the Accumulation of Essential Oil from Endophytic Fungi of Cinnamomum longepaniculatum

Abstract

The aim of this study was to investigate the effect of different inducer on the accumulation of essential oil in C. longepaniculatum endophytic fungus. The effect of the essential oil accumulation in the C. longepaniculatum fungus was studied by adding different inducer with an C. longepaniculatum endophytic fungus (serial number 2J1) with the ability of producing essential oil. The results show that the accumulation of essential oil can be greatly promoted by adding CaSO4 1.5 mg/L, H2O2 9 mmol/L, SA 15 mg/L and MnSO4 3 mmol/L in the PDB medium; compared with CK in the same condition increased 75.56%, 47.18%, 38.35%, 53.95% respectively; reached 0.0934 mg/L, 0.0783 mmol/L, 0.0736 mmol/L, 0.0819 mg/L; through the orthogonal experiment, the combinations 2.0 mg/L CaSO4, 12 mmol/L H2O2, 5 mg/L SA and 7 mmol/L MnSO4 were significantly different from other combinations; the yield reached 0.1847 mg/L and increased by 247.18%, indicating that adding inducer was an effective way to promote the accumulation of essential oil of endophytic fungi. This provides a reference for the artificial regulation of the secondary metabolites of C. longepaniculatum and the enhancement of subsequent microorganisms.