Effects of different hydrocolloids on the production of bacterial cellulose by Acetobacter xylinum using Hestrin–Schramm medium under anaerobic condition

Abstract

In this study, the effect of hydrocolloids on the efficiency of bacterial cellulose (BC) biosynthesis by Acetobacter xylinum was investigated under anaerobic conditions using Hestrin–Schramm medium. With initial glucose concentration of 50 g/L, there was no BC formation while bacterial growth was still noticed through a decrease in pH, total acidity, and sugar content. However, the addition of xanthan gum (XG), blend of microcrystalline cellulose and carboxymethylcellulose (MCC), and konjac glucomannan (KJ) induced cellulose formation, leading to the presence of BC layers on the top of static fermentation medium. After 13 days of fermentation, HS medium supplemented with 0.2% KJ and 0.2% XG resulted in the highest BC production with dried BC content of 6.97 and 6.52 g/L, respectively. In addition, the thickness of the two samples XG and KJ was in the range of 2.54–3.54 mm, and MCC exhibited lowest effectiveness, regardless of its concentration.