Effects of different hormonal concentrations on damask rose (Rosa damascena Mill.) micro-propagation in liquid tissue culture medium

Abstract

Damask rose (Rosa damascena Mill.) from Rosaceae family is one of the most important medicinal plants in Iran which its secondary metabolites are extensively used in pharmaceutical, flavors and fragrance industries. It is usually propagated by cutting and transplanting. Since this method is time-consuming and has usually some problems therefore, in the current study the effects of different hormonal concentrations were studied on micropropagation of superior genotype Khuzestan in liquid tissue culture medium. The lateral buds were cultured on modified Murashige and Skoog (MS) culture environments treated with different concentrations of hormones Benzyl Adenine (BA) and Indole-3-butyric acid (IBA). Results of Means comparison showed that the BA concentration of 0.75 mg.l -1 and IBA concentration of 0.1 mg.l -1 had the highest effect on both shoot number with mean number of 4 and height with mean 2 cm. Also, the effects of NAA and IBA hormones on root number were significant. The best culture medium for increasing root number and percentage was found to be ½ MS with a BA concentration of 0.01 mg.l-1 and IBA concentration of 1 mg/l in combination with AgNO3 concentration of 58.85 µmol.l -1 . The top proliferation treatment was determined at a combination of 0.75 mg.l-¹ BA and 0.1 mg.l-¹ IBA. The best treatment of rooting percent (55%) was determined to be a combination of 0.01 mg.l-¹ BA and 1 mg.l-¹ IBA in presence of 58.85 µmol.lˉ¹ AgNO3.