Abstract
Banana (Musa spp.) breeding has been a slow developing process, due to the absence of seeds and low propagation rates. Selection of valuable cultivars and micro-propagation are promising techniques to accelerate the cultivation process. Therefore, callus culture was carried out, aiming the establishment of plant regeneration protocol that might be used in banana breeding programs. Sword suckers were used as explants, whereas vigorously grown apical shoots obtained from initial in vitro germinated seedlings were sub-cultured in six different MS media at pH 5.8, supplemented with MS Minerals + MS Vitamins + 36.7*FeNaEDTA + 3% sucrose + 0.65% agar (+) different hormonal conditions. Among the different concentrations tested within the experiment, MS Media (MS Minerals + MS Vitamins + 36.7*FeNaEDTA + 3% sucrose + 0.65% agar) with hormones 1 TDZ + 10 IBA showed the highest shoot proliferation and a high rate of multiplication. Even so, there were no significant differences observed for root initiation and plantlet establishment among the six tested medium.
Highlights
Edible bananas (Musa spp.) are the major staple food for rural and urban consumers in the tropical and subtropical countries and an important source of rural income (Rowe, 1981)
Establishment of sub-cultures After 21 days, vigorously grown apical shoots obtained from initial in vitro germinated seedlings were sub-cultured in six different Murasige and Skoog (MS) media at pH 5.8, supplemented with MS Minerals + MS Vitamins + 36.7*FeNaEDTA + 3% sucrose + 0.65% agar (+) different hormonal conditions, as follows: - Medium 1 (M1): (+) 10 Indole-3butyric acid (IBA) + 10 Kinetin, - Medium 2 (M2): (+) 10 BA, - Medium 3 (M3): (+) 10 BA + 1TDZ, - Medium 4 (M4): no hormones, - Medium 5 (M5): (+) 1 TDZ - Medium 6 (M6): (+) 1 TDZ + 10 IBA
The creamy white apical meristem slices turned green when placed in MS medium supplemented with 5 ppm BA and small shoot buds were visible within 21 days of the experiment
Summary
Edible bananas (Musa spp.) are the major staple food for rural and urban consumers in the tropical and subtropical countries and an important source of rural income (Rowe, 1981). Banana has been conventionally propagated through suckers, which represent a time-consuming method, with lower rates of multiplication. To overcome these aspects, the standardized protocols for shoot-tip culture have been commercially exploited by various tissue culture protocols. Tissue culture has been proven as a potential technology to produce millions of identical plantlets, which are disease free and true to parental type. Through this technology, from a single shoot tip or an axillary bud ‘Sagar’), a large quantity of uniform and disease free plants, with good genetic potential, were produced within a short time (Vuylsteke and Langhe, 1985; Wong, 1986)
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