Abstract
<p id="C3">A two-year positioning field experiment was conducted to determine the reasonable regulation measures of selenium (Se) fertilizer for improving wheat grain Se nutrition, and to explore the feasibilities of different Se application approaches in Se agronomic biofortification of wheat grown in the typical Se-deficient soils of Yongshou County, Shaanxi Province. To study the effects of different forms and application methods of Se fertilizers on wheat yield, Se concentration and its accumulation and utilization, with the goal of producing wheat grain with Se concentration of 100 μg kg <sup>-1</sup>, five Se application rates of 0, 15, 18, 700, and 45 g hm<sup>-2</sup> were calculated for the treatments of no Se application (control), soil- and foliar-sodium selenate, and soil- and foliar-sodium selenite in the first year, respectively. In the following wheat season, for further investigating the residual Se availability, no Se fertilizer was applied and each plot was divided into straw removal and straw return. These results showed that the wheat grain yield and straw biomass were not influenced by Se forms and application methods. For the first wheat season, grain Se concentration reached the expected target value with a range of 109-397 μg kg <sup>-1</sup>, and the flour Se concentration varied from 101 μg kg <sup>-1</sup> to 356 μg kg <sup>-1</sup> for all the Se application treatments. In the next year, both grain and white flour Se concentrations were 100 μg kg <sup>-1</sup> higher than under the treatment of soil-sodium selenite, and there was no significant difference of straw removal and straw return. Grain Se biofortification index was 4.7, 16, 0.3, 8.0 (μg kg<sup>-1</sup>) (g hm<sup>-2</sup>)<sup>-1</sup> for soil- and foliar-sodium selenate, and soil- and foliar-sodium selenite, respectively. The Se use efficiency was the highest for foliar-sodium selenate (7.3%), whereas its cumulative use efficiency was only 0.3% in soil-sodium selenite treatment with the long-term residue effects. At wheat harvest, the highest soil available Se was observed for soil-sodium selenite, with 91 μg kg <sup>-1</sup> and 107 μg kg <sup>-1</sup> for the straw removal and straw return, respectively. In conclusion, both soil- and foliar-sodium selenate /selenite were beneficial for producing wheat grain with target Se concentration of 100 μg kg <sup>-1</sup>, and the sodium selenite requirement was the highest, and its residual availability should be taken into consideration for Se biofortification in wheat production in the Se-deficient area of Chinese Loess Plateau.
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