Abstract
This study aimed to determine the effects of different forage combinations on invitro gas production (GP) kinetics, ruminal and milk fatty acid profiles. Forty-five lactating cows were randomly arranged into three groups and fed three total mixed rations (TMRs) with different forage combinations: TMR1, 23% alfalfa hay, 7% Chinese wild ryegrass hay and 15% whole corn silage; TMR2, 30% corn stover plus 15% whole corn silage; TMR3, 30% rice straw plus 15% whole corn silage. In vitro dry matter disappearance ranked: TMR1>TMR2>TMR3, and highest cumulative GP and asymptotic GP occurred in TMR1 while no difference occurred between TMR2 and TMR3. The average GP rate ranked: TMR1>TMR2>TMR3. TMR1 in comparison with TMR2 and TMR3 presented lower rumen contents of acetate and butyrate and greater rumen contents of propionate, valerate, C13:0, C14:0, C15:0, C18:1cis-9, C18:2n-6, C18:3n-3, C20:0 and C22:0 as well as milk C18:2n-6 and C18:3n-3 proportions. Transfer efficiencies of C18:2n-6 and C18:3n-3 from diet to milk ranked: TMR1>TMR2>TMR3. The findings suggest TMRs containing alfalfa hay and Chinese wild ryegrass hay in comparison with corn stover or rice straw improve rumen fermentation and transfer efficiency of C18:2n-6 and C18:3n-3.
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