Effects of different foliar nitrogen fertilizers on cellular nitrogen metabolism and biomass of two shrub willow cultivars

Abstract

We examined the effects of foliar supplementation of different nitrogen sources (urea, Nitamin, NH4NO3, and arginine) to study their efficacy as fertilizers for growth of two clonally propagated shrub willow cultivars; namely, ‘Fish Creek’ (Salix purpurea L.) and ‘Preble’ (Salix viminalis L. × (Salix sachalinensis F. Schmidt × Salix miyabeana Seemen)). Our objectives were to determine (i) if foliar nitrogen application is an effective method of fertilization for the two shrub willows and (ii) if different nitrogen sources are metabolized similarly by the plants. The analyses involved soluble leaf polyamines, amino acids, total protein, total nitrogen and carbon, and plant biomass in response to short-term treatments with four sources of nitrogen. The effects of foliar nitrogen application on leaf chemistry, biomass, and foliar nitrogen content varied according to the form of nitrogen used. The data indicate that (i) urea is the most suitable nitrogen source for foliar spray (29% higher N accumulation vs. Nitamin), whereas arginine is the least suitable, and (ii) different nitrogen sources are metabolized differently by the plant. While the foliar nitrogen application method could become a practical and sustainable way to fertilize shrub willows and other short-rotation biofuel crops, it may also help reduce nitrogen loss to the environment.