Abstract
Summary Current in vitro fertilisation (IVF) practice requires synchronisation between the environment of cultured oocytes and embryos and the surroundings to what they would have been exposed to in vivo. Commercial, sequential media follow this requirement but their exact composition is not available. We have compared two widely used IVF culture media systems using the two choriocarcinoma cell lines JEG-3 and BeWo. The two hormones hCG and progesterone were determined in the culture supernatants as endpoints. In both cell lines, but in a more pronounced way in JEG-3, progesterone rather than hCG production was stimulated, and a higher hormone release was observed in the fertilisation than in the cleavage media. Differences between manufacturers were small and did not favour one system over the other. We conclude that both sequential media systems can be equally well used in current IVF laboratory practice.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
