Abstract
Stem cell treatment is based on Melatonin which is crucial for lots of pathological and physiological pathways. Our aim is determining the most appropriate dose of melatonin affecting the rat adipose tissue mesenchymal stem cells. Stem cells were isolated from male rat adipose tissue. Differentiation and characterization experiments were performed. Cell viability analyses in stem cells were used the XTT [2,3-Bis-(2-methoxy-4-Nitro-5-Sulfophenyl)-2H-Tetrazolium-5-Carboxanilide] assay. After 24h incubation, different concentrations (0.5, 1, 5, 10, 50µM) of extract were treated to the stem cells for 24h, 48 and 72h considering time and dose dependent manner. Total antioxidant status (TAS) and the total oxidant status (TOS) in control cells and melatonin treated cells (5, 10µM) were determined Rel Assay commercial kits. In 24h, melatonin increased cell viability in all groups. When we evaluate the effect of melatonin in 48h, the most proliferation increase was seen at 5, 10µM doses. When the total oxidant activity melatonin was found to be significantly lower in 5 and 10µM dose groups of melatonin. Melatonin increases the survivor of stem cells and the most effective dose is 5 and 10µM. The reduction of the oxidative stress index as a result of treating melatonin to mesenchymal stem cells showed that melatonin is a powerful antioxidant for stem cells.
Published Version
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