Effects of different dental alloys on cytotoxic and apoptosis related genes expression of mouse fibroblast cells L929

Abstract

To investigate effects of the leaching liquids of 5 different kinds of dental alloys on L929 cells at cell level and molecular level. The fibroblast L929 cells of mouse were cultivated in vitro in leaching liquids of 5 different kinds of dental alloys, Au alloy (n = 8), Ag-Pt alloy (n = 8), Co-Cr alloy (n = 8), Ni-Cr alloy (n = 8), and Cu alloy (n = 8). The RPMI 1640 cell medium containing 10% fetal beef serum was used as control. The cytotoxicities of the 5 dental alloys were evaluated by means of methyl thiazolyl tetrazolium (MTT), and the effects of these alloys on the expression of caspase-3, caspase-8, and caspase-9 mRNA of L929 cells were examined using reverse transcription polymerase chain reaction (RT-PCR) method. After 48 hours culture the cytotoxicity of Cu alloy group was in Grade 4 and those of the other groups were all in Grade 0. The mRNA levels of caspase-8 had no change in all groups (P > 0.05). The mRNA levels of caspase-3 were as follows: Cu alloy (0.474 +/- 0.001), the negative control (0.527 +/- 0.003), Au alloy (0.528 +/- 0.013), Co-Cr alloy (0.615 +/- 0.007), Ag-Pd alloy (0.673 +/- 0.009), and Ni-Cr alloy (0.803 +/- 0.037). The mRNA levels of caspase-9 were as follows: Cu alloy (0.532 +/- 0.041), Au alloy (0.574 +/- 0.013), the negative control (0.578 +/- 0.010), Co-Cr alloy (0.617 +/- 0.009), Ag-Pd alloy (0.703 +/- 0.018), and Ni-Cr alloy (0.811 +/- 0.037). There were significant differences between the groups except the negative control group and Au alloy group. The Cu alloy shows the highest cytotoxicity, and the leaching liquids of 5 different kinds of dental alloys may induce cell apoptosis through mitochondrion pathway.