Effects of different concentrations of parecoxib on proliferation and apoptosis of colon cancer LoVo cells

Abstract

Objective To investigate the effects of different concentrations of parecoxib on proliferation and apoptosis of colon cancer LoVo cells.Methods The colon cancer LoVo cells were inoculated in cuhure plate and cultured for 24 h.The cells were randomly divided into 4 groups (n =6 each):control group (C group) and different concentrations of parecoxib groups (P1-3 groups).The cells were incubated with parecoxib 10,40 and 160μmol/L for 24 h in P1-3 groups respectively.The rates of proliferation inhibition were measured by MTT assay.The colony formation rates were measured by colony formation assay.The apoptotic rate was determined by flow cytometry.The Survivin and caspase-3 mRNA expression in the cells was detected by RT-PCR.Results Compared with C group,the rates of proliferation inhibition and apoptotic rate were significantly increased,the colony formation rates were significantly decreased,the expression of Survivin mRNA was down-regulated,and the expression of caspase-3 mRNA was up-regulated in a concentration-dependent manner in P1-3 groups (P ＜ 0.05).Conclusion Parecoxib can inhibit the proliferation of LoVo cells and induce the apoptosis in LoVo cells in a concentration-dependent manner through down-regulating the expression of Survivin mRNA and up-regulating the expression of caspase-3 mRNA. Key words: Cyclooxygenase inhibitors; Colonic neoplasms; Cell proliferation; Apoptosis