Effects of different concentrations of artificial joint prosthesis nanoparticles on cytotoxicity and osteogenic differentiation of bone marrow stromal cell (BMSCs) in vivo

Abstract

To explore the effects of artificial joint prosthesis nanoparticles on cytotoxicity and osteogenic differentiation of Bone Marrow Stromal Cell (BMSCs). SD rats were used to isolate cells and titanium metal obtained from artificial joint revision surgery was prepared into nanoparticles by the direct current arc plasma method. The SD rats were then divided into control group, blank group (group A: D-MEM medium+10% FBS), low concentration group (group B: 0.01 mg/mL titanium alloy particles), medium concentration group (group C: 0.05 mg/mL titanium alloy particles), high concentration group (group D: 0.1 mg/mL titanium alloy particles plus D-MEM complete medium+10% FBS), followed by analysis of cytotoxicity and osteogenic differentiation of BMSCs. Among the three test groups, group B showed highest percentage of viable cells and group D had lowest. The percentages of viable cells in three test groups were significantly lower and cell death rate were higher than those in the control group (P < 0.05). Titanium alloy metal particles inhibited osteogenic differentiation of BMSCs in a dose dependent manner. The cells in control group were stained and dark brown nuclei were visible. The stained cells were almost invisible in group A, and less red-stained areas in group B, and with a small amount of cell nuclei. Artificial joint prosthesis particles had certain effects on cytotoxicity and osteogenic differentiation of BMSCs in vivo. Among them, the high concentration of titanium alloy nanoparticles test group had the highest percentage of viable cells.