Effects of different combinations of prescription of invigorate blood and diffuse Bì formula on myocardial protection of hyperlipidemia rats with isoproterenol-induced ischemic injury

Abstract

This research aimed at observing the effects of different combinations of prescription ofinvigorate blood and diffuse Bi formula (IBDB) on resisting myocardial ischemic injury and adjusting blood lipid to seek for the best combination for IBDB. The research used the model of hyperlipidemia rats with isoproterenol-induced myocardial ischemic injury and treated by gavage with 8 different combinations of IBDB using orthogonal design to observe the effects of different combinations on electrocardiogram (ECG), heart function, Superoxide dismutase (SOD), malondialdehyde (MDA) and total antioxidation capacity (T-AOC) of serum and myocardium and blood lipid of acute ischemia and thus find out the best combination with mathematical method. The result indicated that different combinations of IBDB could protect ischemic myocardium and the heart function of rats and directly work against myocardial injury and adjust blood lipid at different levels. Comparison between all treated groups suggests that the 7th, 6th and 3rd combinations are superior than the 4th and 5th in improving ECG (P<0.05) and the 7th and 8th are superior than the 1st in improving FS (P<0.05), the 2ndis superior than the 5th and 7th in increasing SOD of serum (P<0.05), the 7th combination is superior in decreasing MDA of serum and myocardium and every combination except the 8thcan reduce CHO without significant difference. Through the comparison of prescription effects, it is found out that Combination 7 is the best for IBDB. Key words: Invigorate blood and diffuse Bi formula, combination, myocardial ischemic injury, selection from direct comparison, Traditional Chinese Medicine.