Abstract
Conventional fluorescent lamps that are used in tissue culture are costly light sources, exhibiting excessive wavelength emission-bandwidth that must be replaced by alternative, less costly, and much lower power-consuming energy sources. The use of Light-Emitting Diodes (LEDs) is the best option due to their potential role as elicitors of secondary metabolite production in many plant models. Gynura procumbens (G. procumbens) is widely used for treating various diseases. Here, leaf explants were cultivated in MS medium that was supplemented with 0.5 mg/L of naphthaleneacetic acid (NAA) and 2.0 mg/L of benzylaminopurine (BAP) for 30 days under white, blue, and red LEDs. Secondary metabolites were analyzed by High Performance Liquid Chromatography (HPLC) and Liquid Chromatography-Mass Spectrometry (LC-MS). Blue LEDs elicited the highest antioxidant activity, total flavonoid, and phenolic content. Furthermore, the content of cyanidin-monoglucosides significantly increased under blue light.
Highlights
Light is one of the most crucial environmental factors that affect the developing plant and regulate its behavior [1]
Of benzylaminopurine (BAP) (Supplementary Table S1). This condition showed that the highest antioxidant activity (57.90 ± 2.32), total flavonoid content (TFC, 0.29 ± 0.14 μg/g), and total phenol content (TPC, 0.97 ± 0.03 mg/g) were observed in the Murashige Skoog (MS) basal medium that was supplemented with the same combination, at a statistically significant level (Supplementary Figure S1)
We found method showed that blue light was the most effective in increasing anthocyanin accumulation with cyanidin-monoglucoside in the leaves of G. procumbens and determined its concentration by High Performance Liquid Chromatography (HPLC)
Summary
Light is one of the most crucial environmental factors that affect the developing plant and regulate its behavior [1]. Fluorescent lamps, high-pressure sodium lamps, and metal halide lamps are used as the light sources for in vitro culture. They contain unnecessary radiation wavelengths that lead to low quality radiation for the stimulation of growth and they are reportedly responsible for as much as 65% of the total electricity consumed in tissue culture laboratories [2,3,4]. Research has shown that it is mostly white, red, and blue light wavelengths that increase signal transduction and betalain biosynthesis [7]. It is estimated that almost 90% of plant development and physiology is influenced by the absorption of blue and red light (LEDs) [8,9].
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