Effects of dietary vitamin C on tissue ascorbate and collagen status in sturgeon hybrids (Acipenser ruthenus L. x Acipenser baeri Brandt)

Abstract

Summary The aim of this study was to determine the vitamin C status of various tissues, and collagen concentration in cartilage, of a sterlet (Acipenser ruthenus L.) x Siberian sturgeon (Acipenser baerii Brandt) hybrid fed different dietary vitamin C rations. Growth, vitamin C status and collagen concentration were measured in groups fed diets supplemented with 150, 300, 600 mg.kg-1 ascorbate-2-polyphosphate (APP); 100, 200, 400 mg.kg-1 ascorbate-2-monophosphate (AMP), 1000 mg.kg-1 L-ascorbic acid (AA) and with an almost ascorbate free diet (Total AA > 5 mg.kg-1), as a control. No significant differences in growth were observed, and no external symptoms of scurvy developed in the vitamin C-free group. No reduction in total vitamin C concentration was revealed in the tissues examined, as compared with initial concentrations. Ascorbate-2-phosphate esters were only found in kidney or hepatopancreas of fish fed with these vitamin C derivatives. Significantly (p<0.05) lower contents of collagen were observed in the control group after 8 weeks feeding. No significant differences were found among the groups after 16 weeks although the collagen concentration in the cartilage was slightly lower in the control group without vitamin C supplementation.