Effects of dietary Vibrio sp. 33 on growth, innate immunity, gut microbiota profile and disease resistance against Vibrio splendidus of juvenile sea cucumber Apostichopus japonicus

Abstract

To investigate the ability of Vibrio sp. V33 supplementation on the growth performance, innate immunity, intestinal microbiota, and disease resistance of the juvenile sea cucumber Apostichopus japonicus, a feeding experiment was conducted. Our results revealed that dietary Vibrio sp. V33 could significantly enhanced sea cucumber growth rate, and the immune parameters including total coelomocytes counts (TCC), phagocytosis, respiratory burst, immune-related enzyme activities (acid phosphatase, alkaline phosphatase, superoxide dismutase, catalase, superoxide dismutase, and nitric oxide synthetase) were all markedly improved in coelomocytes of sea cucumbers fed with V33 (P < 0.05). Furthermore, the composition of the bacterial community in the intestinal contents of the sea cucumber was surveyed by 16S rRNA sequencing. Beta diversity analysis indicated that the bacterial compositions of sea cucumbers were significantly different between V33 and Control groups. At the phylum level, Proteobacteria, Actinobacteria, Firmicutes, and Bacteroidetes were the most prevalent phyla in sea cucumber gut microbiota. The abundance of Firmicutes (20.58%), Bacteroidetes (9.77%), and Verrucomicrobia (3.04%) were significantly higher in V33 group when compared with Control. Moreover, genus Mycobacterium was markedly decreased to 0.5% after V33 feeding, while the abundance of genus Rhodococcus was significantly increased by 6.9-fold (P < 0.01) under the same condition, indicating V33 diet might promotes the colonization of beneficial bacteria in the gut of sea cucumber. After Vibrio splendidus challenge, the survival rate of juvenile sea cucumbers fed with V33 diet was significantly higher than that fed with Control diet. All our current results suggested that the Vibrio sp. V33 could used as a probiotic for healthier production of sea cucumbers in aquaculture.