Abstract

Fourteen midlactation Holstein cows were used in an 80-d study to examine supplementation strategies during intensive rotational grazing. Factors examined were the concentration of protein in the supplement [12 or 16% crude protein (CP) on a dry matter basis] and the amount of supplement offered (6.4 or 9.6kg/d per cow). The supplement was offered in equal portions three times daily after milking. Pasture, composed of alfalfa (Medicago sativa) and orchardgrass (Dactylis glomerata), was divided into 27 0.16-ha paddocks that were grazed for 12 to 24h. Intake of forage tended to increase as CP in the supplement increased but was unaffected by the amount of supplement offered. Intakes of organic matter and dry matter and the digestion of these nutrients in the total tract increased as CP in the supplement increased and as the amount of supplement increased. Ruminal pH and concentrations of volatile fatty acids were unaffected by treatments, but concentrations of NH3 N increased as CP in the supplement increased. An increase in CP resulted in a greater intake and flow of total N to the duodenum. The flow of microbial N to the duodenum and the efficiency of microbial protein synthesis were unaffected by treatment. Flows of total amino acids and essential amino acids to the duodenum tended to increase as CP in the supplement increased. Yield of fat-corrected milk and concentrations of fat and protein in milk were unaffected by treatment. These results suggested that the supply and digestion of nutrients in grazing dairy cows may be improved through an increase in the CP concentration of the supplement or the amount of supplement offered. However, effects on the yield of milk and milk components may be small.

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