Effects of dietary organic acids and probiotics on laying performance, egg quality, serum antioxidants and expressions of reproductive genes of laying ducks in the late phase of production

Abstract

Five hundred and forty Cheery Valley ducks were used to investigate the effects of dietary supplementation of acidifier and compound probiotics, individually or in combination, on production performance, egg quality, immune and oxidative status, expression of reproductive, and calcium binding related genes from 42 wk to 48 wk of age. Ducks were randomly allocated to 9 treatment groups with 6 replicates and 10 ducks per replicate for each group. A 3 × 3 factorial arrangement, with 3 dietary inclusion levels of acidifier and probiotics (0, 2, and 3 g/kg acidifier; 0, 1, and 2 g/kg probiotics) were used. The acidifier used was mainly consisted of Benzoic acid, Fumaric acid, phosphoric acid, and formic acid. The main components of the probiotics were Bacillus subtilis and Clostridium butyricum. Dietary supplementation of probiotics improved the daily feed intake, egg production rate, and body weight of ducks (P < 0.05), and diet acidifier also increased the daily feed intake compared to the control (P < 0.01). Egg quality was improved by diet inclusion of probiotics, including Haugh unit, albumen height, egg shape index (P < 0.01), and eggshell hardness (P = 0.05). A significant increase in Haught unit and yolk weight was observed in ducks fed diet added with acidifier (P < 0.05). Acidifier supplementation reduced the total antioxidant capacity (T-AOC), immunoglobulin A (IgA), and IgG content and the catalase (CAT) activity in the serum (P < 0.05), in accompanied with an increased malondialdehyde (MDA) concentration (P < 0.05). Serum total superoxide dismutase (T-SOD) activities were improved by dietary inclusion of probiotics (P < 0.05). There was an interaction effects on serum IgA and IgG contents between acidifier and probiotics (P < 0.05). Diet supplementation of probiotics improved the ovary follicle-stimulating hormone receptor (FSHR) and estrogen receptor (ER) gene expressions (P < 0.01), while dietary acidifier reduced the transcription levels of FSHR and luteinizing hormone receptor (LHR) (P < 0.01) in ovary. In the uterus of the oviduct, expressions of FSHR, and carbonic anhydrase 2 (CA2) were also increased by diet probiotics (P < 0.01), and diet acidifier reduced the gene expressions of calbindin-D28k (CaBP-D28k) and CA2 (P < 0.05). Significant interaction effects between diet acidifier and probiotics were obtained on gene expressions of FSHR, LHR, and ovalbumin (OVAL) in the ovary (P < 0.05), and LHR, CaBP-D28k, and CA2 (P < 0.05) in the uterus. It can be concluded that production performance and egg quality of laying ducks can be improved in the late phase of reproduction by dietary inclusion of probiotics, while the organic acid mixture caused a decline in serum antioxidant and immune capacity of the ducks.