Effects of dietary Mitracarpus scaber leaves extract on growth, physiological, antioxidants, and mucosal immune profiles of North African catfish, Clarias gariepinus, and resistance against Edwardsiella tarda infection.

Abstract

This study evaluated the effect of Mitracarpus scaber leaves extract (MSLE) as a dietary supplement on growth performance, physiological, antioxidants, and mucosal immune profiles of North African catfish, Clarias gariepinus, and resistance to Edwardsiella tarda infection. Four isonitrogenous diets containing 0, 2, 4, or 6 MSLE g/kg were fed to North African catfish fingerlings (n = 240; mean weight = 5.80g) for 56days. After the feeding trial, 10 fish from each experimental tank were intraperitoneally injected with E. tarda (ATCC 15,947; 1.0 × 108CFU/mL) while clinical signs and mortalities were observed for 14days. The results show that North African catfish fed dietary MSLE had better growth performance, physiological antioxidants, and mucosal immune profiles than when fed a control diet. The best growth, physiological, antioxidant, and mucosal immune response profiles were noticed in the fish fed diet containing 6 MSLE g/kg. Furthermore, fish fed 6 MSLE g/kg diet had a higher survival rate after E. tarda infection. These results indicated that supplementation of MSLE at 6g/kg improves growth, physiological, antioxidant, and mucosal immune profiles, and promotes resistance of North African catfish.