Effects of Dietary Lysine on Growth Performance, Serum Concentrations of Insulin-Like Growth Factor-I (IGF-I) and IGF-I mRNA Expression in Growing Rabbits

Abstract

The effects of dietary lysine on production performance, serum concentrations of metabolites, growth hormone (GH), insulin-like growth factor-I (IGF-I) and IGF-I mRNA expression in growing rabbits were examined. One hundred weaned New Zealand rabbits were allocated to individual cages and randomly offered a diet containing 5.5 (L1), 6.5 (L2), 7.5 (L3), 8.5 (L4), or 9.5 g (L5) lysine per kg diet. The results showed that the average daily gain (ADG) of the rabbits from L3, L4 or L5 was higher than those from L1 or L2 ( P < 0.05). The feed gain ratio (F/G) in the rabbits from L4 or L5 was lower than those from L1 or L2 ( P < 0.05). Dietary lysine did not affect serum concentrations of total protein (TP), glucose, insulin (INS), and growth hormone (GH) ( P > 0.05). The quadratic effects of lysine on the serum urea nitrogen (SUN) concentration was detected ( P = 0.035). Serum IGF-I concentrations had a trend to increase quadratically with the increasing dietary lysine ( P = 0.07). A significant correlation was found between serum IGF-I concentrations ( x, ng mL −1) and ADG ( y, g kg −1): y = −0.017 x 2 + 1.984 x + 20.87 ( R 2 = 0.8982, P = 0.003). The relative abundance of hepatic and muscular IGF-I mRNA tended to increase with increasing dietary lysine levels ( P = 0.053 and 0.082, respectively). Providing the diets mainly consisted of corn, wheat bran and peanut vine, the most appropriate dietary lysine level for growing meat rabbits from weaning to 70 d old was found to be 8.5 g kg −1, and IGF-I may be an important factor controlling growth of weaned rabbits.