Abstract
Two feeding trials were conducted to evaluate the effects of heat‐killed Lactobacillus plantarum (HK‐LP) on larval and post‐larval kuruma shrimp, Marsupenaeus japonicus Bate. Five microbound diets were formulated to contain levels of a preparation containing 20% HK‐LP (HK‐LP Prep): 0, 0.001, 0.01, 0.1, and 1 g/kg. In the first experiment, zoea1 stage larvae were fed test diets for 8 d. Some parameters such as survival, developmental stage, metamorphosis to post‐larvae, and formalin stress resistance were evaluated. In second trial, post‐larval shrimp (16 ± 0.04 mg) were fed test diets for 30 d. Survival, body weight gain, specific growth rate, feed efficiency ratio, individual dry weight, total body length, osmotic and formalin stress resistances, and protease activity were evaluated. Results indicated that the larvae that received HK‐LP Prep at 0.1 and 1 g/kg diets showed significantly (P < 0.05) higher survival than that of the control group. For the post‐larval trial, shrimp that received HK‐LP Prep at 1 g/kg diet were significantly higher than the control group in most of the growth parameters and stress resistance. However, shrimp that received HK‐LP Prep at 0.1 g/kg diet were significantly higher than the control group in survival only. For protease activity, there was no significant difference detected among groups.
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