Effects of dietary green tea (Camellia sinensis L.) supplementation on growth performance, lipid metabolism, and antioxidant status in a sturgeon hybrid of Sterlet (Huso huso ♂×Acipenser ruthenus ♀) fed oxidized fish oil.

Abstract

Lipid content of diet is very susceptible to oxidation, especially when stored for a long time, so for evaluating protective effects of green tea in fish received oxidized oil, this study was done. Lipid content of diet was replaced by oxidized fish oil (OFO) in 0, 50, and 100%. Green tea extract (GTE) was added to diet in three levels, 0, 5, and 100mg/kg giving a total of nine experimental diets. Two hundred and seventy sturgeon hybrid of Sterlet (Huso huso ♀×Acipenser ruthenus ♂) with initial weight of 212.6±0.7g after 2weeks adaptation randomly divided in 27 fiberglass tanks with 700L volume. Fish were fed satiated three times daily. After 6weeks, biometry was done to evaluate growth performance and blood samples were taken for biochemical analysis. The result showed that feeding with oxidized oil had no effects on growth. However, in fish fed GTE, growth indices improved slightly. Feeding with OFO reduced serum total cholesterol, triacylglycerol, and low-density lipoprotein, while increased high density lipoprotein. Dietary GTE moderated the effects of OFO on lipid metabolism. Feeding with the OFO increased activity of serum superoxide dismutase, catalase, glutathione peroxidase, and malondialdehyde contents. In fish received both OFO and GTE, reduced activity of serum antioxidant enzymes and malondialdehyde content was recorded in compare to fish fed only OFO. According to the result of the present study, it can be argued that feeding of sturgeon hybrid of Sterlet with OFO has negative effects on lipid metabolism and antioxidant status, whereas GTE dosages used in this study have protective effects on fish from the adverse effects of oxidized oil.