Abstract
Microbiota affects host health and plays an important role in dysbiosis. The study examined the effect of diet including grape seed meal (GSM) with its mixture of bioactive compounds on the large intestine microbiota and short-chain fatty acid synthesis in weaned piglets treated with dextran sodium sulfate (DSS) as a model for inflammatory bowel diseases. Twenty-two piglets were included in four experimental groups based on their diet: control, DSS (1 g/kg/b.w.+control diet), GSM (8% grape seed meal inclusion in control diet), and DSS+GSM (1 g/kg/b.w., 8% grape seed meal in control diet). After 30 days, the colon content was isolated and used for microbiota sequencing on an Illumina MiSeq platform. QIIME 1.9.1 pipeline was used to process the raw sequences. Both GSM and DSS alone and in combination affected the diversity indices and Firmicutes:Bacteroidetes ratio, with significantly higher values in the DSS-afflicted piglets for Proteobacteria phylum, Roseburia, Megasphera and CF231 genus, and lower values for Lactobacillus. GSM with high-fiber, polyphenol and polyunsaturated fatty acid (PUFA) content increased the production of butyrate and isobutyrate, stimulated the growth of beneficial genera like Prevotella and Megasphaera, while countering the relative abundance of Roseburia, reducing it to half of the DSS value and contributing to the management of the DSS effects.
Highlights
The intestinal inflammatory bowel disease (IBD) affects the life quality of a large number of people and is a significant problem for public health [1,2,3]
HPLC-DAD–MS analysis showed that grape seed meal (GSM) was rich in flavonoids, the highest concentration being observed for caffeoylquinic acid
Our results showed that GSM, a common by-product of grape seed oil processing, which contains significant concentrations of several bioactive compounds, like polyphenols, polyunsaturated fatty acid (PUFA), fibers, minerals, etc., had a selective modulatory effect on several bacterial genera in the colon of pigs challenged with dextran sodium sulfate (DSS)
Summary
The intestinal inflammatory bowel disease (IBD) affects the life quality of a large number of people and is a significant problem for public health [1,2,3]. It is known that IBDs are symptoms of an unbalanced inflammatory response between commensal microflora, pathogens, and the host immune system [4], the precise nature of the intestinal microbiota perturbation and the resulting effects remains to be identified. Most of the risk factors implicated in the development of IBD, including diet, stress and anti-inflammatory drugs, can perturb the Grapeseed Meal Effect on Piglets Microbiota commensal component of the microbiota [5, 6]. While the microbiota of healthy hosts shows little shifts in time, the gut microbiota of IBD affecting hosts is not stable. There is growing interest to manipulate the gut microbiota for preventative and therapeutic purposes
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.