Effects of dietary fish oil replacement with blended vegetable oils on growth, lipid metabolism and antioxidant capacity of subadult swimming crabPortunus trituberculatus

Abstract

A 120-day trial was conducted to assess the effects of dietary fish oil replacement with vegetable oils on growth, lipid metabolism and antioxidant capacity of subadult swimming crab Portunus trituberculatus. Five isonitrogenous and isolipidic diets were formulated to replace 0, 250, 500, 750 and 1000g/kg of fish oil with a mixture of soybean and rapeseed oil (defined as D1-D5), and each treatment had 30 replicate crabs. Dietary fish oil replacement had no significant effects on growth of the crabs, while the D3 had the highest hepatosomatic index and total lipids in hepatopancreas. The triglyceride and lipase activities in hepatopancreas increased significantly with increasing dietary fish oil replacement. The D4 had the highest levels of alkaline phosphatase (ALP) and acid phosphatase (ACP) in the hepatopancreas, as well as the haemolymph ALP, ACP and peroxidase. The highest levels of haemolymph total antioxidant capacity, catalase and malondialdehyde were detected in D1. Total n-3 polyunsaturated fatty acids levels in hepatopancreas decreased significantly with increasing dietary fish oil replacement. In conclusion, dietary fish oil replacement had no significant effects on growth of P. trituberculatus, and 500g/kg of fish oil replacement could improve antioxidant capacity, but excessive replacement level will enhance lipid accumulation and peroxidation in the hepatopancreas.